What kind of effects do Fe2O3 and Al2O3 nanoparticles have on anaerobic digestion, inhibition or enhancement?

Abstract

Fe₂O₃ and Al₂O₃ nanoparticles are widely used in products and find their way to wastewater treatment plants through the contact of water with these products. In this study, impacts of Fe₂O₃ and Al₂O₃ nanoparticles on methane potential of waste activated sludge (WAS) were investigated by comparing long and short term toxicity test results, modelling and FISH analysis. Methane production from the samples treated with the maximum concentration of Fe₂O₃ nanoparticles decreased 28.9% at the end of the long term BMP test. EC₅₀ value for BMP test of the Fe₂O₃ nanoparticles was calculated as 901.94 mg/gTS with high coefficient of determination. Methane production from the samples treated with Al₂O₃ nanoparticles increased up to 14.8% (p > 0.05) at the end of the BMP test. However, short term toxicity tests for Fe₂O₃ and Al₂O₃ nanoparticles showed no impact on anaerobic digestion of WAS. Kinetic parameters obtained from models and captured FISH images were consistent with these results. Different impacts of nanoparticles on methane production suggested that anaerobic microorganisms can be affected from nanoparticles in various mechanisms. Hydrolysis (k_H) and overall reaction rates (k_R) values were determined as 0.0277 and 0.1441 d^-1, respectively for each concentration of Al₂O₃ nanoparticles and raw WAS. Similarly, methane production from WAS containing 5, 50, 150 and 250 mgFe₂O₃/gTS were modeled with same kinetic values. However, k_H constant was calculated as 0.0149 d^-1 for 500 mgFe₂O₃/gTS. This means that Fe₂O₃ nanoparticles starting from this concentration inhibited the methanogenic consortium and caused decreased biogas production and spesific methane production rate.

Keywords: Anaerobic digestion; Inhibition; Modeling; Nanoparticle; Sewage sludge.