Characterization of self-assembled hybrid siloxane-phosphocholine bilayers

Mark B Frampton; Michael H L Nguyen; Mitchell DiPasquale; Robert Dick; Drew Marquardt; Paul M Zelisko

doi:10.1016/j.chemphyslip.2018.07.007

Characterization of self-assembled hybrid siloxane-phosphocholine bilayers

Chem Phys Lipids. 2018 Nov:216:1-8. doi: 10.1016/j.chemphyslip.2018.07.007. Epub 2018 Aug 9.

Authors

Mark B Frampton¹, Michael H L Nguyen², Mitchell DiPasquale², Robert Dick³, Drew Marquardt⁴, Paul M Zelisko⁵

Affiliations

¹ Department of Chemistry and Centre for Biotechnology, Brock University, St. Catharines, Ontario, Canada; Advanced Biomanufacturing Centre, Brock University, St. Catharines, Ontario, Canada. Electronic address: mframpton@brocku.ca.
² Department of Chemistry and Biochemistry, University of Windsor, Windsor, Ontario, Canada.
³ Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, United States.
⁴ Department of Chemistry and Biochemistry, University of Windsor, Windsor, Ontario, Canada. Electronic address: drew.marquardt@uwindsor.ca.
⁵ Department of Chemistry and Centre for Biotechnology, Brock University, St. Catharines, Ontario, Canada; Advanced Biomanufacturing Centre, Brock University, St. Catharines, Ontario, Canada. Electronic address: pzelisko@brocku.ca.

PMID: 30098944
DOI: 10.1016/j.chemphyslip.2018.07.007

Abstract

We have synthesized six new hybrid siloxane phosphocholines (SiPCs) and examined their self-assembly behaviour in aqueous dispersions. Employing small angle X-ray scattering we have characterized SiPC bilayers. SiPCs exhibit differential self-assembly behaviour that results from the interplay between the siloxane fatty acid in the sn-2 position and the differing chain length fatty acids in the sn-1 position. SiPCs that possess a fatty acid chain of a C₈-C₁₄ chain length in the sn-1 position form unilamellar vesicles. Extending the fatty acid chain length to C₁₆ and C₁₈ allows for the formation of both unilamellar and multilamellar vesicles. We propose that the preferential formation of unilamellar vesicles is the result of an enhanced hydrophobic effect imparted by siloxane chains at the termini of lipid tails.

Keywords: Differential scanning calorimetry; Liposomes; Siloxane-phospholipid; Small angle X-ray scattering; Unilamellar vesicles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Hydrophobic and Hydrophilic Interactions
Lipid Bilayers / chemical synthesis*
Lipid Bilayers / chemistry
Molecular Structure
Phosphorylcholine / chemistry*
Siloxanes / chemistry*

Substances

Lipid Bilayers
Siloxanes
Phosphorylcholine