A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins

Jelena Bogdanović Pristov; Ivan Spasojević

doi:10.1007/978-1-4939-8745-0_18

A One-Step Staining Protocol for In-Gel Fluorescent Visualization of Proteins

Methods Mol Biol. 2018:1853:149-158. doi: 10.1007/978-1-4939-8745-0_18.

Authors

Jelena Bogdanović Pristov¹, Ivan Spasojević²

Affiliations

¹ Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia. jelenabog@gmail.com.
² Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia. redoxsci@gmail.com.

PMID: 30097940
DOI: 10.1007/978-1-4939-8745-0_18

Abstract

Native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)-PAGE are among the most frequently applied techniques in protein analysis. Here we describe a fast one-step method for fluorescent visualization of proteins. Following PAGE, gels are soaked in solution of potassium ferricyanide (100 mM) in 1 M NaOH, and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method is comparable with standard Coomassie Brilliant Blue staining.

Keywords: Electrophoresis; Ferricyanide; Fluorescence; PAGE; Staining protocol; Tryptophan.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophoresis, Gel, Two-Dimensional* / methods
Fluorescent Dyes* / chemistry
Image Processing, Computer-Assisted
Optical Imaging
Proteins* / analysis
Proteins* / chemistry
Staining and Labeling* / methods

Substances

Fluorescent Dyes
Proteins