Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases

Linda A Stevens; Joel Moss

doi:10.1007/978-1-4939-8588-3_10

Mono-ADP-Ribosylation Catalyzed by Arginine-Specific ADP-Ribosyltransferases

Methods Mol Biol. 2018:1813:149-165. doi: 10.1007/978-1-4939-8588-3_10.

Authors

Linda A Stevens¹, Joel Moss²

Affiliations

¹ Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
² Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA. mossj@nhlbi.nih.gov.

PMID: 30097866
DOI: 10.1007/978-1-4939-8588-3_10

Abstract

Methods are described for determination of arginine-specific mono-ADP-ribosyltransferase activity of purified proteins and intact cells by monitoring the transfer of ADP-ribose from NAD⁺ to a model substrate, e.g., arginine, agmatine, and peptide (human neutrophil peptide-1 [HNP1]), and for the nonenzymatic hydrolysis of ADP-ribose-arginine to ornithine, a noncoded amino acid. In addition, preparation of purified ADP-ribosylarginine is included as a control substrate for ADP-ribosylation reactions.

Keywords: ADP-ribose; ADP-ribosylarginine; Defensins; HNP-1; Mono-ADP-ribosylation; NAD+.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

ADP Ribose Transferases / chemistry
ADP Ribose Transferases / genetics
ADP Ribose Transferases / isolation & purification*
ADP-Ribosylation / genetics*
Adenosine Diphosphate Ribose / analogs & derivatives
Adenosine Diphosphate Ribose / chemistry
Adenosine Diphosphate Ribose / genetics
Adenosine Diphosphate Ribose / isolation & purification*
Arginine / chemistry
Catalysis
Humans
Molecular Biology / methods*
Ornithine / chemistry

Substances

Adenosine Diphosphate Ribose
Arginine
ADP-ribosylarginine
Ornithine
ADP Ribose Transferases