QueE: A Radical SAM Enzyme Involved in the Biosynthesis of 7-Deazapurine Containing Natural Products

Methods Enzymol. 2018:606:95-118. doi: 10.1016/bs.mie.2018.05.001. Epub 2018 Jul 13.

Abstract

7-Carboxy-7-deazaguanine (CDG) is a common intermediate in the biosynthesis of 7-deazapurine-containing natural products. The biosynthesis of CDG from GTP requires three enzymes: GTP cyclohydrolase I, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) synthase, and CDG synthase (QueE). QueE is a member of the radical S-adenosyl-l-methionine (SAM) superfamily and catalyzes the SAM-dependent radical-mediated ring contraction of CPH4 to generate CDG. This chapter focuses on methods to reconstitute the activity of QueE in vitro.

Keywords: 7-Deazapurine; CDG synthase; Radical SAM; tRNA modification.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Bacillus subtilis / metabolism
  • Bacterial Proteins / isolation & purification*
  • Bacterial Proteins / metabolism
  • Biocatalysis
  • Biological Products / metabolism*
  • Carbon-Nitrogen Lyases / isolation & purification*
  • Carbon-Nitrogen Lyases / metabolism
  • Free Radicals / metabolism
  • Purines / metabolism*
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • S-Adenosylmethionine / metabolism

Substances

  • 7-deazapurine
  • Bacterial Proteins
  • Biological Products
  • Free Radicals
  • Purines
  • Recombinant Proteins
  • S-Adenosylmethionine
  • Carbon-Nitrogen Lyases