Development of DNA aptamer-based sensor for electrochemical detection of C-reactive protein

Abstract

C-reactive protein (CRP) is a crucial biomarker of cardiovascular diseases and for its detection both optical and electrochemical techniques were applied. This study concerns the application of DNA aptamer as recognition layer for CRP detection. For that purpose aptamer immobilization method on gold surface was selected and the content of receptor layer was optimized to ensure an efficient binding to target protein. The quality of the monolayer was verified by the application of chronocoulometry and atomic force microscopy. Using thiolated aptamers provided the formation of layers of highest density and stability. The square-wave voltammetry experiments performed in the presence of methylene blue redox indicator revealed a linear response of aptasensor towards CRP in the range from 1 to 100 pM. Moreover, a DNA aptamer - based sensor showed good selectivity towards C-reactive protein in comparison to interfering proteins including BSA and IgE. Finally, the analysis of CRP in serum sample was conducted using the developed aptasensor.

Keywords: C-reactive protein; DNA aptamer; Redox indicator; Self-assembled monolayers; Voltammetry.