Preliminary evaluation of BioFire FilmArray® Gastrointestinal Panel for the detection of noroviruses and other enteric viruses from wastewater and shellfish

Environ Sci Pollut Res Int. 2018 Sep;25(27):27657-27661. doi: 10.1007/s11356-018-2869-2. Epub 2018 Aug 7.

Abstract

The BioFire FilmArray® Gastrointestinal Panel was evaluated for the rapid detection of adenovirus, astrovirus, norovirus, rotavirus and sapovirus from influent and effluent wastewater and shellfish. The multiplex BioFire FilmArray® Gastrointestinal Panel compared well to singleplex qPCR/RT-qPCR methods for the detection of adenovirus, astrovirus, rotavirus and sapovirus from influent and effluent wastewater samples. However, the BioFire FilmArray® Gastrointestinal Panel showed poor performance for the detection of norovirus, significantly underestimating its presence in wastewater and shellfish samples when compared with the singleplex norovirus GI and GII RT-qPCR assays. Therefore, improvement on detection efficiency for norovirus from environmental and food samples is necessary before using results from the FilmArray® Gastrointestinal Panel to assess associated public health risks.

Keywords: BioFire FilmArray® Gastrointestinal Panel; Enteric viruses; Norovirus; Rapid detection.

MeSH terms

  • Adenoviridae / isolation & purification*
  • Antigens, Viral / analysis
  • Feces / virology
  • Gastroenteritis / virology
  • Humans
  • Molecular Diagnostic Techniques
  • Norovirus / isolation & purification*
  • Real-Time Polymerase Chain Reaction
  • Rotavirus / isolation & purification*
  • Sapovirus / isolation & purification*
  • Shellfish / virology*
  • Wastewater / virology*

Substances

  • Antigens, Viral
  • Waste Water