Limited ERG11 Mutations Identified in Isolates of Candida auris Directly Contribute to Reduced Azole Susceptibility

Antimicrob Agents Chemother. 2018 Sep 24;62(10):e01427-18. doi: 10.1128/AAC.01427-18. Print 2018 Oct.

Abstract

Multiple Erg11 amino acid substitutions were identified in clinical isolates of Candida auris originating from India and Colombia. Elevated azole MICs were detected in Saccharomyces cerevisiae upon heterologous expression of C. aurisERG11 alleles that encoded for Y132F or K143R substitutions; however, expression of alleles encoding I466M, Y501H, or other clade-defined amino acid differences yielded susceptible MICs. Similar to other Candida species, specific C. aurisERG11 mutations resulted directly in reduced azole susceptibility.

Keywords: Candida auris; ERG11; antifungal resistance; azoles; reduced azole susceptibility.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Azoles / pharmacology*
  • Candida / drug effects*
  • Candida / genetics*
  • Cytochrome P-450 Enzyme System / genetics
  • Drug Resistance, Fungal / genetics
  • Microbial Sensitivity Tests
  • Mutation / genetics*

Substances

  • Antifungal Agents
  • Azoles
  • Cytochrome P-450 Enzyme System