Effect of thermoultrasound on aflatoxin M1 levels, physicochemical and microbiological properties of milk during storage

Abstract

The aim of this research was to determine the physicochemical properties, microbial counts and aflatoxin M₁ (AFM₁) levels of thermoultrasonicated, pasteurized and untreated milk (control) at days 1, 7 and 14 of storage. Thermoultrasound treatment was performed at a rate of 20 kHz for 10 or 15 min and 95% amplitude on homogenized and non-homogenized milk samples. Results showed that most physicochemical parameters were within the Mexican norms established for milk. Ultrasound treatment for 15 min reduced solids precipitation (p < 0.05) in unhomogenized milk during storage as compared to the pasteurized milk. All samples complied with aerobic mesophilic counts limits set by the Mexican norm except the control and the homogenized milk sample which was thermoultrasonicated for 10 min. Enterobacteriaceae counts of pasteurized and 15 min-thermoultrasound homogenized milks complied with the norm. The lowest levels of AFM₁ were found in the 10 min-thermoultrasound unhomogenized milk (0.15 ± 0.05 pg AFM⁠₁E/mL) one day after storage. Thermoultrasound did not affect the color of samples but homogenized milk treated for 10 min exhibited less total color difference. A high phenolic content was found in thermoultrasound and pasteurized milks on day 1. Thermoultrasound could be an alternative to milk pasteurization that preserves the physicochemical and microbiological quality of milk while reducing AFM₁ levels.

Keywords: Aflatoxin M(1); Antioxidant; Color; Microbiological; Milk; Physical stability; Thermoultrasound.