Abstract
This communication reports on a possible distinct role of HMGB1 protein. Biophysical studies revealed that HMGB1 binds and stabilizes the G-quadruplex of the KRAS promoter element that is responsible for most of the transcriptional activity. Biological data showed that inhibition of HMGB1 increases KRAS expression. These results suggest that HMGB1 could play a role in the gene transcriptional regulation via the functional recognition of the G-quadruplex.
MeSH terms
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Cell Line, Tumor
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Fluorescein / chemistry
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Fluorescent Dyes / chemistry
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G-Quadruplexes*
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Gene Expression Regulation
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HMGB1 Protein / genetics
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HMGB1 Protein / metabolism*
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Humans
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Promoter Regions, Genetic
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Protein Binding
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Proto-Oncogene Proteins p21(ras) / genetics
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Proto-Oncogene Proteins p21(ras) / metabolism*
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RNA, Small Interfering / genetics
Substances
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Fluorescent Dyes
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HMGB1 Protein
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HMGB1 protein, human
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KRAS protein, human
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RNA, Small Interfering
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Proto-Oncogene Proteins p21(ras)
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Fluorescein