Succinic acid biosynthesis from cane molasses under low pH by Actinobacillus succinogenes immobilized in luffa sponge matrices

Abstract

Succinic acid (SA) production by Actinobacillus succinogenes 130Z using cane molasses as a low cost carbon source was developed. With molasses pretreated by 150 kDa membrane, the highest SA concentration (45.6 g/L), productivity (1.27 g/L·h) and yield (0.76 g SA/g sugars) were obtained under an optimal pH 6.4, which were increased by 1.04 folds compared to those with model sugar mixture due to the effect of vitamins in molasses. Meanwhile, the ratio of sugars in the cane molasses had little effect on SA production. To further enhance SA productivity, the cells were immobilized in luffa sponge matrices (LSM), and repeated batch cultures were carried out for 5 cycles, demonstrating a stable and reliable long-term performance. Compared with the batch culture, the SA productivity enhanced by 49.6% in the LSM system with repeated batch culture. These results suggest that the cell immobilization approach is promising for industrial applications.

Keywords: Actinobacillus succinogenes; Cane molasses; Luffa sponge matrices; Repeated batch culture; Succinic acid.