Abstract
We describe a label-free approach based on Raman spectroscopy, to study drug-induced apoptosis in vivo. Spectral-shifts at wavenumbers associated with DNA, proteins, lipids, and collagen have been identified on breast and melanoma tumor tissues. These findings may enable a new analytical method for rapid readout of drug-therapy with miniaturized probes.
MeSH terms
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Animals
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Antibodies / immunology
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Antineoplastic Agents / pharmacology
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Apoptosis / drug effects*
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Breast Neoplasms / metabolism*
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Caspase 3 / immunology
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Caspase 3 / metabolism
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Doxorubicin / pharmacology
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Immunohistochemistry
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Intercalating Agents / pharmacology
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Melanoma / metabolism*
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Mice, Nude
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Spectrum Analysis, Raman / methods*
Substances
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Antibodies
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Antineoplastic Agents
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Intercalating Agents
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Doxorubicin
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Casp3 protein, mouse
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Caspase 3