Ultrasound Parameters for Human Osteoarthritic Subchondral Bone ex Vivo: Comparison with Micro-Computed Tomography Parameters

Wataru Kiyan; Yasuaki Nakagawa; Akira Ito; Hirotaka Iijima; Kohei Nishitani; Momoko Tanima-Nagai; Shogo Mukai; Junichi Tajino; Shoki Yamaguchi; Akihiro Nakahata; Jue Zhang; Tomoki Aoyama; Hiroshi Kuroki

doi:10.1016/j.ultrasmedbio.2018.06.003

Ultrasound Parameters for Human Osteoarthritic Subchondral Bone ex Vivo: Comparison with Micro-Computed Tomography Parameters

Ultrasound Med Biol. 2018 Oct;44(10):2115-2130. doi: 10.1016/j.ultrasmedbio.2018.06.003. Epub 2018 Jul 29.

Authors

Affiliations

¹ Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan; Researching Department, Furuno Electric Company, Ltd., Nishinomiya, Japan.
² Department of Orthopaedic Surgery, National Hospital Organization Kyoto Medical Center, Kyoto, Japan.
³ Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
⁴ Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan; Department of System Design Engineering, Keio University, Yokohama, Japan.
⁵ Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
⁶ Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA.
⁷ Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan; Department of Physical Therapy, School of Health Sciences at Narita, International University of Health and Welfare, Narita, Chiba, Japan.
⁸ Department of Motor Function Analysis, Human Health Sciences, Graduate School of Medicine, Kyoto University, Kyoto, Japan. Electronic address: kuroki.hiroshi.6s@kyoto-u.ac.jp.

PMID: 30064850
DOI: 10.1016/j.ultrasmedbio.2018.06.003

Abstract

The aim of this study was to identify ultrasound parameters reflecting subchondral porosity (P_o), subchondral plate thickness (T_pl) and bone volume fraction at the trabecular bone region (BV/TV_Tb). Sixteen osteoarthritic human lateral femoral condyles were evaluated ex vivo using a 15-MHz pulsed-echo ultrasound 3-D scanning system. The cartilage-subchondral bone (C-B) surface region (layer 1) and inner subchondral bone region (layer 2) were analyzed; we newly introduced entropy (ENT) and correlation (COR) of ultrasound texture parameters of the parallel (x) or perpendicular (z) direction to the C-B interface for this analysis. P_o, T_pl and BV/TV_Tb were evaluated as reference measurements using micro-computed tomography. ENT_L1x (ENT of layer 1, x-direction) and ENT_L1z were significantly correlated with P_o (both r values = 0.58), COR_L2x with T_pl (r = -0.73) and COR_L2z with BV/TV_Tb (r = -0.66). These are efficient indicators of the characteristics of osteoarthritis-related subchondral bone; the other texture parameters were not significant.

Keywords: Bone volume fraction; Cartilage; Micro-computed tomography; Osteoarthritis; Porosity; Subchondral bone; Texture parameter; Thickness; Ultrasound.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Aged
Cancellous Bone / diagnostic imaging
Cancellous Bone / pathology
Cancellous Bone / surgery
Female
Humans
Imaging, Three-Dimensional / methods*
Knee Joint / diagnostic imaging
Knee Joint / pathology
Knee Joint / surgery
Male
Osteoarthritis, Knee / diagnostic imaging*
Osteoarthritis, Knee / pathology
Osteoarthritis, Knee / surgery
Reproducibility of Results
Ultrasonography / methods*
X-Ray Microtomography / methods*