Glucoraphanin and sulforaphane evolution during juice preparation from broccoli sprouts

Abstract

Broccoli sprouts are considered functional food as they are naturally enriched in glucoraphanin (GR) that is the biological precursor of the anticancer compound sulforaphane (SFN). Due to its health promoting value, also broccoli sprout juice is becoming very popular. The present study aimed to quantitatively assess the conversion of GR to its hydrolysis products, SFN and SFN-nitrile, during the juice preparation process. We demonstrated that SFN plus SFN-nitrile yield from glucoraphanin is quite low (≈25%) and that some SFN is lost during the juice preparation partially due to the spontaneous conversion to sulforaphane-amine or conjugation to GSH and proteins naturally present in the juice. Our results demonstrate that the detection of the sole SFN free form does not provide reliable information about the real concentration of this functional compound in the juice.

Keywords: Bioactive molecules; Broccoli sprouts; Glucoraphanin (PubChem CID: 9548634); Glucosinolate; Isothiocyanates; Sulforaphane; Sulforaphane (PubChem CID: 5350); Sulforaphane nitrile (PubChem CID: 543743).