Date palm (Phoenix dactylifera L.) is one of the oldest fruit crops in the arid regions of the Middle East. However, little information is available regarding its plastid genomes. In this study, we sequenced the chloroplast (cp) genomes of two economically important but genomically unexplored date palm cultivars of Phoenix dactylifera (var. Naghal and Khanezi). The data assembly and genome annotation revealed a typical quadripartite structure similar to Arecaceae, and the genome sizes of Naghal and Khanezi were 158,210 bp and 158,211 bp, respectively. Structurally, both cp genomes were comprised of four regions: a pair of inverted repeats (27,273 bp for Khanezi and for Naghal 27,272 bp), a large single-copy region (86,090 bp and 86,092 bp) and a small single-copy region (17,575 bp and 17,574 bp). Both genomes had 138 representative genes, whereas 227 and 229 randomly distributed microsatellites were also observed in Khanezi and Naghal, respectively. Phylogenetic analysis based on the whole cp genomes and 68 shared genes showed identical phylogenetic trees of Khanezi and Naghal forming clades with Khalas and Aseel cultivars, respectively. The current study showed detailed comparative cp genome analysis, which could be essential for broader population genetics and molecular studies of these four date palm cultivars.