N-(Aminobutyl)-N-(ethylisoluminol) (ABEI)-functionalized gold nanoparticles (AuNPs) on cobalt disulfide nanowires (ABEI/AuNPs/CoS2 NWs) are rapidly synthesized through a microwave-assisted reduction of chloroauric acid (HAuCl4) on CoS2 NWs with ABEI. The obtained nanohybrids with enhanced chemiluminescence are exploited for the non-enzymatic detection of H2O2.