Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis

Jun Xu; Jin-Woo Lee; Soo-Kyoung Park; Sung-Bok Lee; Young-Hoon Yoon; Sun-Hee Yeon; Ki-Sang Rha; Ji-Ae Choi; Chang-Hwa Song; Yong Min Kim

doi:10.1016/j.clim.2018.07.014

Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis

Clin Immunol. 2018 Dec:197:19-26. doi: 10.1016/j.clim.2018.07.014. Epub 2018 Jul 26.

Authors

Affiliations

¹ Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Republic of Korea; State Key Laboratory of Respiratory Disease, Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, China; Department of Otorhinolaryngology-Head and Neck Surgery, Yanbian University Hospital, Yanji, Jilin, China.
² Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
³ Department of Ophthalmology, Chungnam National University Hospital, Chungnam National University School of Medicine, Daejeon, Republic of Korea.
⁴ Department of Microbiology, College of Medicine, Chungnam National University, Daejeon, Republic of Korea.
⁵ Department of Otorhinolaryngology-Head and Neck Surgery, Research Institute for Medical Science, Chungnam National University School of Medicine, Daejeon, Republic of Korea. Electronic address: entkym@cnu.ac.kr.

PMID: 30056130
DOI: 10.1016/j.clim.2018.07.014

Abstract

B-cell activating factor (BAFF) has been proposed to play a crucial role in the pathogenesis of chronic rhinosinusitis with nasal polyp (CRSwNP). The aim of this study was to evaluate the role of toll-like receptor (TLR) 9-mediated BAFF activation on the pathogenesis of CRSwNP. NP and uncinate tissue (UT) were obtained from patients with CRSwNP or CRS without NP, and control subjects. The expression of TLR9, high mobility group box-1 protein (HMGB1), type I interferon (IFN), BAFF, and anti-double stranded DNA (dsDNA) antibody were examined in the tissues and the cultured dispersed NP cells (DNPCs). The expression of TLR9, HMGB1, type I IFN, BAFF, and anti-dsDNA antibody were elevated in NP tissue compared to the UTs. Exposure to TLR9 agonist increased the type I IFN expression in vitro, which further increased BAFF production. In conclusion, we provided a novel therapeutic potential of TLR9 agonist in CRSwNP.

Keywords: B-cell activating factor; Nasal polyp; Sinusitis; Toll-like receptor; Type I interferon.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Aged, 80 and over
B-Cell Activating Factor / drug effects
B-Cell Activating Factor / genetics*
B-Cell Activating Factor / metabolism
Chronic Disease
Female
Frontal Sinus / metabolism
HMGB1 Protein / genetics*
HMGB1 Protein / metabolism
Humans
In Vitro Techniques
Interferon-alpha / drug effects
Interferon-alpha / genetics
Interferon-alpha / metabolism
Interferon-beta / drug effects
Interferon-beta / genetics
Interferon-beta / metabolism
Male
Middle Aged
Nasal Polyps / metabolism*
RNA, Messenger / metabolism
Rhinitis / metabolism*
Sinusitis / metabolism*
Toll-Like Receptor 9 / agonists
Toll-Like Receptor 9 / metabolism*

Substances

B-Cell Activating Factor
HMGB1 Protein
HMGB1 protein, human
Interferon-alpha
RNA, Messenger
TLR9 protein, human
TNFSF13B protein, human
Toll-Like Receptor 9
Interferon-beta