High-performance electrochemical biosensor for nonenzymatic H2O2 sensing based on Au@C-Co3O4 heterostructures

Hongxia Dai; Yonglei Chen; Xiaoying Niu; Congjie Pan; HongLi Chen; Xingguo Chen

doi:10.1016/j.bios.2018.07.022

High-performance electrochemical biosensor for nonenzymatic H₂O₂ sensing based on Au@C-Co₃O₄ heterostructures

Biosens Bioelectron. 2018 Oct 30:118:36-43. doi: 10.1016/j.bios.2018.07.022. Epub 2018 Jul 12.

Authors

Hongxia Dai¹, Yonglei Chen², Xiaoying Niu³, Congjie Pan³, HongLi Chen³, Xingguo Chen⁴

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Gansu University of Chinese Medicine, Lanzhou 730000, China.
² State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address: chyl@lzu.edu.cn.
³ State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
⁴ State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.

PMID: 30055418
DOI: 10.1016/j.bios.2018.07.022

Abstract

An ingenious and viable method for preparing heterogeneous Co₃O₄ dodecahedrons that contain carbon and encapsulated Au nanoparticles (Au@C-Co₃O₄) was proposed via pyrolysis of Au nanoparticle-encapsulated zeolitic imidazolate framework-67 (Au@ZIF-67). The obtained Au@C-Co₃O₄ possessed hierarchically porous structure, abundant active sites, excellent conductivity, and durability, all of which can significantly improve the analysis performance of the biosensor. Meanwhile, the fabricated electrode based on the porous Au@C-Co₃O₄ showed remarkable electrocatalytic performance towards H₂O₂ with a limit of detection of 19 nM and ultra-high sensitivity of 7553 μA mM^-1 cm^-2, even when the Au content in the composite was as low as 0.85 wt%. This novel biosensor was successfully used to monitor H₂O₂ concentration released from living cells, and the results can be used to identify cancer cells, thus advancing the use of transition metal oxides in the field of biosensing.

Keywords: Au nanoparticles; Co(3)O(4); Hydrogen peroxide; Living cells; Nonenzymatic biosensor.

MeSH terms

Biosensing Techniques / instrumentation
Biosensing Techniques / methods*
Cobalt / chemistry*
Electrochemical Techniques*
Electrodes
Gold / chemistry*
Hydrogen Peroxide / analysis*
Metal Nanoparticles

Substances

Cobalt
Gold
Hydrogen Peroxide