Effect of microencapsulated essential oil form Chamaecyparis obtusa on monocyte-derived dendritic cell activation and CD4+ T cell polarization

PLoS One. 2018 Jul 27;13(7):e0201233. doi: 10.1371/journal.pone.0201233. eCollection 2018.

Abstract

The essential oil of Chamaecyparis obtusa (C. obtusa), which is used in soap, toothpaste, and aromatic agents, has been known to have anti-inflammatory properties. In this study, we investigated the effects of microencapsulated C. obtusa essential oil on airborne fungus-induced dendritic cell (DC) activation and Th immune responses. We stimulated monocyte-derived DCs with Alternaria alternate and lipopolysaccharide (LPS). To determine the anti-inflammatory effects, we pre-treated DCs with various concentrations of microencapsulated C. obtusa essential oil and collected the supernatants to measure interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), and we determined the expression of cell surface molecules. The effects of the essential oil on CD4+ T cells polarization was determine by culturing stimulated DCs and autologous CD4+ T cells. Alternaria enhanced the production of IL-6 and TNF-α from DCs, and pretreating DCs with 0.001, 0.01, and 0.05% of the essential oil significantly inhibited their production. Increased CD80 and CD86 expression by Alternaria was significantly inhibited with 0.05% of the essential oil. Alternaria-induced IL-5, IL-10, and interferon-gamma from CD4+ T cells were significantly inhibited with C. obtusa essential oil in a dose dependent manner. C. obtusa influenced both Alternaria- and LPS-induced Th1 and Th2 polarization of CD4+ T cells. These results suggest a novel pharmacological use for C. obtusa essential oil to treat inflammatory airway diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternaria / chemistry
  • Capsules
  • Chamaecyparis / chemistry*
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Humans
  • Interleukin-6 / immunology
  • Lipopolysaccharides / pharmacology
  • Monocytes / cytology
  • Monocytes / immunology*
  • Oils, Volatile / chemistry
  • Oils, Volatile / pharmacology*
  • Th1 Cells / cytology
  • Th1 Cells / immunology*
  • Th2 Cells / cytology
  • Th2 Cells / immunology*
  • Tumor Necrosis Factor-alpha / immunology

Substances

  • Capsules
  • IL6 protein, human
  • Interleukin-6
  • Lipopolysaccharides
  • Oils, Volatile
  • Tumor Necrosis Factor-alpha

Associated data

  • figshare/10.6084/m9.figshare.6728396

Grants and funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1D1A1B03931622). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.