Metabolomics of Vibrio cholerae

Yusuke Minato; Jay S Kirkwood; Claudia C Häse

doi:10.1007/978-1-4939-8685-9_5

Metabolomics of Vibrio cholerae

Methods Mol Biol. 2018:1839:45-51. doi: 10.1007/978-1-4939-8685-9_5.

Authors

Yusuke Minato¹, Jay S Kirkwood², Claudia C Häse³

Affiliations

¹ Department of Microbiology and Immunology, University of Minnesota Medical School, Minneapolis, MN, USA.
² Department of Pharmaceutics, University of Washington, Seattle, WA, USA.
³ Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA. claudia.hase@oregonstate.edu.

PMID: 30047053
DOI: 10.1007/978-1-4939-8685-9_5

Abstract

Metabolomics is an "omics" approach to quantitatively measure a large set of metabolites. In this chapter, we describe an example method for performing liquid chromatography coupled to mass spectrometry (LC-MS)-based untargeted metabolomics on a cell extract from Vibrio cholerae.

Keywords: Liquid chromatography–mass spectrometry (LC–MS); Phenyl-bonded phase; Q-TOF mass spectrometer; Reversed-phase chromatography; Untargeted metabolomics.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Chromatography, High Pressure Liquid
Chromatography, Liquid
Metabolome*
Metabolomics* / methods
Tandem Mass Spectrometry
Vibrio cholerae / metabolism*