In this review paper, we give a historical perspective of the development of imaging modalities to visualize platelet biogenesis and how this contributed to our current understanding of megakaryopoiesis and thrombopoiesis. We provide some insight how distinct in vivo and in situ imaging methods, including ultramicrographs, have contributed to the current concepts of platelet formation. The onset of intravital microscopy into the mouse bone marrow has markedly modified and challenged our thinking of platelet biogenesis during the last decade. Finally, we discuss ongoing work, which was presented at the recent International Society on Thrombosis and Haemostasis (ISTH) meeting.
Keywords: bone marrow; imaging; megakaryocyte; platelet biogenesis; proplatelets.