Pulsatile motion occurs in the cardiac systolic period and leads to significantly larger displacement of water molecules as it is observed during diffusion weighted image acquisition. Obvious pulsatile motion arises in the brain stem and basal ganglia and might affect the corticospinal tract. So far there is no consensus on the real effect of pulsatile motion on diffusion properties, diffusion tensor parameters and fiber tractography and on the role of cardiac-gating to overcome these effects. The present study aimed at detecting effects of pulsatile motion on imaging properties and reconstruction of the corticospinal tract. Non-gated and cardiac-gated data of 22 healthy subjects was acquired using clinical standard protocols and analysed with regard to effects on signal intensities, diffusion tensor properties and tractography results concerning the corticospinal tract. Analyses resulted in obvious effects of pulsatile motion on signal intensities, especially alterations in diffusion tensor properties, compensated by the application of cardiac-gating, whereas no effect on fiber tract volume was seen. Therefore, pulsatile motion and cardiac-gating should be kept in mind as critical aspects when analysing and interpreting diffusion tensor properties within the human brain, but are of minor interest when considering fiber tractography of the corticospinal tract.