Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula

Myriam Sánchez; Christine Le Signor; Gregoire Aubert; Brigitte Darchy; Karine Gallardo; Richard D Thompson

doi:10.1007/978-1-4939-8633-0_5

Targeting Induced Local Lesions IN Genomes (TILLING) in Medicago truncatula

Methods Mol Biol. 2018:1822:71-82. doi: 10.1007/978-1-4939-8633-0_5.

Authors

Myriam Sánchez¹, Christine Le Signor¹, Gregoire Aubert¹, Brigitte Darchy¹, Karine Gallardo¹, Richard D Thompson²

Affiliations

¹ INRA, University of Bourgogne Franche-Comté, Agroécologie, AgroSup Dijon, F-21000 Dijon, France.
² INRA, University of Bourgogne Franche-Comté, Agroécologie, AgroSup Dijon, F-21000 Dijon, France. richard.thompson@inra.fr.

PMID: 30043297
DOI: 10.1007/978-1-4939-8633-0_5

Abstract

TILLING is a reverse genetics strategy that combines the high density of point mutations provided by traditional chemical mutagenesis with rapid screening of DNA pools from a mutagenized population for induced mutations (McCallum et al., Nat Biotechnol 18:455-457, 2000). This high-throughput technique allows the identification of point mutations in any gene of interest.

Keywords: EMS chemical mutagenesis; Medicago truncatula; Reverse genetics; TILLING.

MeSH terms

Ethyl Methanesulfonate / pharmacology
Genome, Plant*
Genomics* / methods
Medicago truncatula / drug effects
Medicago truncatula / genetics*
Mutagenesis / drug effects
Nucleic Acid Amplification Techniques
Phenotype
Polymorphism, Single Nucleotide
Reproducibility of Results
Seeds / drug effects
Seeds / genetics

Substances

Ethyl Methanesulfonate