Albumin coated copper-cysteine nanozyme for reducing oxidative stress induced during sperm cryopreservation

Bioorg Chem. 2018 Oct:80:621-630. doi: 10.1016/j.bioorg.2018.07.020. Epub 2018 Jul 18.

Abstract

In the present work, SOD mimetic nanozyme (NACu-Cys) consisting of Cu-Cys complex and nano-albumin (NA) were synthesized. After characterizing the nanozyme, its superoxide dismutase (SOD) behavior was evaluated by inhibition of the pyrogallol autoxidation method. The results revealed that NACu-Cys exhibited SOD mimetic activity with a half inhibition concentration (IC50) value of 7.0 × 10-3 µM and a turnover number (kcat) of 5.4 × 107 s-1. In the next step, this nanozyme was applied as a protective agent against oxidative stress induced by sperm cryopreservation. Increasing the motility, raising the viability and reducing the apoptosis occurred as a result of NACu-Cys additions to human sperm freezing medium. Comparison between the natural SOD and SOD mimic behavior of NACu-Cys revealed that this nanoparticle has the ability to be used as oxidative stress decrescent during cryopreservation process.

Keywords: Copper-cysteine complex; Cryopreservation; Nano-albumin; Nanonzyme; Superoxide dismutase mimetic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Biomimetic Materials / metabolism*
  • Cattle
  • Copper / metabolism*
  • Cryopreservation / methods
  • Cysteine / metabolism*
  • Humans
  • Male
  • Oxidative Stress / drug effects
  • Semen Preservation / methods*
  • Serum Albumin, Bovine / metabolism*
  • Spermatozoa / cytology
  • Spermatozoa / metabolism*
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Serum Albumin, Bovine
  • Copper
  • Superoxide Dismutase
  • Cysteine