This paper intended to explore the effect of alkaline H₂O₂ pretreatment on the biodegradability and the methane generation potential of greenhouse crop waste. A multi-variable experimental design was implemented. In this approach, initial solid content (3⁻7%), reaction time (6⁻24 h), H₂O₂ concentration (1⁻3%), and reaction temperature (50⁻100 °C) were varied in different combinations to determine the impact of alkaline H₂O₂ pretreatment. The results indicated that the alkaline H₂O₂ pretreatment induced a significant increase in the range of 200⁻800% in chemical oxygen demand (COD) leakage into the soluble phase, and boosted the methane generation potential from 174 mLCH₄/g of volatile solid (VS) to a much higher bracket of 250⁻350 mLCH₄/gVS. Similarly, the lignocellulosic structure of the material was broken down and hydrolyzed by H₂O₂ dosing, which increased the rate of volatile matter utilization from 31% to 50⁻70% depending on selected conditions. Alkaline H₂O₂ pretreatment was optimized to determine optimal conditions for the enhancement of methane generation assuming a cost-driven approach. Optimal alkaline H₂O₂ pretreatment conditions were found as a reaction temperature of 50 °C, 7% initial solid content, 1% H₂O₂ concentration, and a reaction time of six h. Under these conditions, the biochemical methane potential (BMP) test yielded as 309 mLCH₄/gVS. The enhancement of methane production was calculated as 77.6% compared to raw greenhouse crop wastes.
Keywords: alkaline H2O2 pretreatment; breakdown of lignocellulosic structure; greenhouse crop waste; methane generation; process optimization.