Black-odor rivers have become a prominent environmental problem, especially for developing countries. A laboratory experiment was conducted to determine the optimum operating parameters of artificial aquatic plants (AAP) to provide a theoretical and scientific basis for their application in black-odor rivers. The purification mechanism of operating parameters for AAP was also explored at the micro-organic and genetic levels by high-throughput sequencing. Chemical oxygen demand (COD) and ammonia nitrogen (NH4+-N) were measured in systems with different AAP lengths and pH. After 24 days, the best removal efficiencies of APP for COD and NH4+-N were 90.07 and 82.40% for 100 cm and 90.70 and 91.90% for pH values of 8.0-9.0, respectively. High-throughput sequencing analysis revealed that the relative abundance of Flavobacterium in the AAP was 7.80% at 50 cm, while the proportion increased to 29.30% at 100 cm. The abundance of microorganisms improved continuously with increased length, and the ratio of Acinetobacter increased obviously at pH 8.0-9.0 relative to pH 6.0-7.0. Furthermore, the AAP were used in Qihe Artificial Wetland in Shandong Province, China. The results revealed that the average removal efficiencies of AAP for COD and NH4+-N were 27.75 and 14.34%, respectively, in the artificial wetland. Therefore, AAP was beneficial to the growth of bacteria and could be used in the treatment of black-odor rivers.
Keywords: Artificial aquatic plants; Black-odor rivers; High-throughput sequencing; Purification mechanism; Removal efficiency.