The roles of Arabidopsis proteins of Lhcb4, Lhcb5 and Lhcb6 in oxidative stress under natural light conditions

Abstract

Under light conditions, highly reactive oxygen species (ROS) can be generated in the antenna systems and the reaction center of photosystems (PS). The protective roles of Lhcb4 (CP29), Lhcb5 (CP26) and Lhcb6 (CP24), three minor chlorophyll binding antenna proteins during photoinhibition have been well studied. However, their regulatory mechanisms against oxidative damages under natural light conditions remain unknown. Here we investigated their specific roles in oxidative stress responses and photosynthetic adaptation by using the Arabidopsis thaliana knockout lines grown in the field condition. All three mutant lines exhibited decreased energy-transfer efficiency from the LHCII (light-harvesting complex II) to the PSII reaction center. Oxygen evolution capacity decreased slightly in the plants lacking Lhcb4 (koLHCB4) and Lhcb6 (koLHCB6). Photosynthetic rates and fitness for the plants lacking Lhcb5 (koLHCB5) or koLHCB6 grown in the field were affected, but not in the plants lacking Lhcb4. Antioxidant analysis indicated the lowest antioxidant enzyme activities and the lowest levels of non-enzymatic antioxidants in koLHCB6 plants. In addition, koLHCB6 plants accumulated much higher levels of superoxide and hydrogen, and suffered more severe oxidative-damages in the field. Our results clearly demonstrate that Lhcb6 may be involved in alleviating oxidative stress and photoprotection under natural conditions.

Keywords: Antioxidant enzymes; Arabidopsis thaliana; Chlorophyll binding antenna proteins; Chlorophyll fluorescence; Photosystem II; Reactive oxygen species.