Selective and sensitive fluorescent and colorimetric chemosensor for detection of CO32- anions in aqueous solution and living cells

Şükriye Nihan Karuk Elmas; Furkan Ozen; Kenan Koran; Ahmet Orhan Gorgulu; Gokhan Sadi; Ibrahim Yilmaz; Serkan Erdemir

doi:10.1016/j.talanta.2018.06.036

Selective and sensitive fluorescent and colorimetric chemosensor for detection of CO₃^2- anions in aqueous solution and living cells

Talanta. 2018 Oct 1:188:614-622. doi: 10.1016/j.talanta.2018.06.036. Epub 2018 Jun 15.

Authors

Şükriye Nihan Karuk Elmas¹, Furkan Ozen², Kenan Koran³, Ahmet Orhan Gorgulu³, Gokhan Sadi⁴, Ibrahim Yilmaz⁵, Serkan Erdemir⁶

Affiliations

¹ Karamanoglu Mehmetbey University, Kamil Ozdag Faculty of Science, Department of Chemistry, Karaman 70100, Turkey.
² Akdeniz University, Faculty of Education, Department of Mathematics and Science, Antalya, Turkey.
³ Firat University, Faculty of Science, Department of Chemistry, Elazıg 23119, Turkey.
⁴ Karamanoglu Mehmetbey University, Kamil Ozdag Faculty of Science, Department of Biology, Karaman 70100, Turkey.
⁵ Karamanoglu Mehmetbey University, Kamil Ozdag Faculty of Science, Department of Chemistry, Karaman 70100, Turkey. Electronic address: iyilmaz@kmu.edu.tr.
⁶ Selcuk University, Faculty of Science, Department of Chemistry, Konya 42075, Turkey.

PMID: 30029421
DOI: 10.1016/j.talanta.2018.06.036

Abstract

A new colorimetric and fluorescent chemosensor for visual determination of carbonate ions was developed by the microwave assisted solvent free synthesis of 7,8-dihydroxy-3-(4-methylphenyl) coumarin (DHMC). The structural characterization of DHMC was confirmed by microanalysis and spectroscopy methods (MALDI-TOF, FT-IR, ¹H NMR, ¹³C NMR, and 2D HETCOR). The binding behaviors of DHMC were investigated towards various anions by UV-vis and fluorescence spectroscopy. DHMC showed a selective and sensitive fluorometric and colorimetric responses towards carbonate ion over other anions. The detection limit of CO₃^2- was found to be 1.03 µM. Moreover, the fluorescence imaging in living cells suggests that DHMC has a great potential in the biological imaging application. It has been demonstrated that DHMC can be used as a rapid and reliable sensor for the determination of carbonate anion in a variety of practical applications.

Keywords: Carbonate; Chemosensor; Coumarin; Fluorescent sensor; Molecular sensing.

MeSH terms

Carbonates / analysis*
Carbonates / metabolism*
Colorimetry / methods*
Coumarins / chemical synthesis
Coumarins / chemistry
Coumarins / pharmacology*
Fluorescence
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry
Fluorescent Dyes / pharmacology*
Limit of Detection
Models, Chemical
Quantum Theory
Saccharomyces cerevisiae / metabolism
Spectrometry, Fluorescence / methods*

Substances

Carbonates
Coumarins
Fluorescent Dyes