Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus

Zhao Wang; Bing Huang; Milton Thomas; Chithra C Sreenivasan; Zizhang Sheng; Jieshi Yu; Ben M Hause; Dan Wang; David H Francis; Radhey S Kaushik; Feng Li

doi:10.1016/j.virol.2018.07.013

Detailed mapping of the linear B Cell epitopes of the hemagglutinin (HA) protein of swine influenza virus

Virology. 2018 Sep:522:131-137. doi: 10.1016/j.virol.2018.07.013. Epub 2018 Jul 18.

Authors

Zhao Wang¹, Bing Huang², Milton Thomas¹, Chithra C Sreenivasan¹, Zizhang Sheng³, Jieshi Yu¹, Ben M Hause⁴, Dan Wang⁵, David H Francis⁶, Radhey S Kaushik⁷, Feng Li⁸

Affiliations

¹ Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA.
² Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA; Poultry Research Institute, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.
³ Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
⁴ Cambridge Technologies, Oxford Street Worthington, MN 56187, USA.
⁵ Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA; BioSNTR, Brookings, SD 57007, USA.
⁶ Department of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD 57007, USA.
⁷ Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA. Electronic address: radhey.kaushik@sdstate.edu.
⁸ Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA; BioSNTR, Brookings, SD 57007, USA. Electronic address: feng.li@sdstate.edu.

Abstract

Using the Hemagglutinin (HA) protein peptide array of H1N1 pdm09 and a panel of swine antisera against various swine influenza H1 and H3 clusters, we identified three immunoreactive epitopes with one (peptide 15) located in HA1 (amino acids 57-71) and two (peptides 121 and 139) in HA2 (amino acids 481-495 and 553-566). Further analysis showed that all swine antisera of H1 clusters efficiently recognized two HA2 epitopes; peptides 121 and 139, with only a subset of antisera reactive to HA1-derived peptide 15. Interestingly, none of these peptides were reactive to SIV H3 antisera. Finally, intranasal inoculation of peptides 15 and 121 into pigs revealed that peptide 121, not peptide 15, was able to generate antibody responses in some animals. The results of our experiments provide an important foundation for further analyzing the immune response against these peptides during natural viral infection and also provide peptide substrates for diagnostic assays.

Keywords: B cell epitope; H1 subtype; H3 subtype; Hemagglutinin (HA) Protein; Peptide; Swine influenza.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Antibodies, Viral / blood
Antibodies, Viral / immunology
Epitope Mapping*
Epitopes, B-Lymphocyte / analysis*
Epitopes, B-Lymphocyte / immunology*
Hemagglutinin Glycoproteins, Influenza Virus / immunology*
Influenza A Virus, H1N1 Subtype / immunology*
Orthomyxoviridae Infections / immunology
Orthomyxoviridae Infections / veterinary
Swine
Swine Diseases / immunology

Substances

Antibodies, Viral
Epitopes, B-Lymphocyte
H1N1 virus hemagglutinin
Hemagglutinin Glycoproteins, Influenza Virus

Abstract

Publication types

MeSH terms

Substances

Grants and funding