Morphologic and molecular characterization of Brachonella pulchra (Kahl, 1927) comb. nov. (Armophorea, Ciliophora) with comments on cyst structure and formation

William Bourland; Johana Rotterová; Ivan Čepička

doi:10.1099/ijsem.0.002888

Morphologic and molecular characterization of Brachonella pulchra (Kahl, 1927) comb. nov. (Armophorea, Ciliophora) with comments on cyst structure and formation

Int J Syst Evol Microbiol. 2018 Sep;68(9):3052-3065. doi: 10.1099/ijsem.0.002888. Epub 2018 Jul 20.

Authors

William Bourland¹, Johana Rotterová², Ivan Čepička²

Affiliations

¹ 1Department of Biological Sciences, Boise State University, Boise, ID 83725-1515, USA.
² 2Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic.

PMID: 30028289
DOI: 10.1099/ijsem.0.002888

Abstract

In this article we provide morphologic and morphometric data based on in vivo observation, protargol impregnation, scanning electron microscopy and an 18S rRNA gene sequence for another member of the genus Brachonella, Brachonella pulchra comb. nov. (basionym: Metopus pulcher Kahl, 1927). We also provide preliminary data on resting cyst structure and formation in Brachonella pulchra and discuss the possible taxonomic usefulness of these structures.

Keywords: 18S rRNA gene; Anaerobic ciliates; Metopus; Protargol impregnation; extrusome; phylogeny.

MeSH terms

Ciliophora / classification*
Microscopy, Electron, Scanning
Phylogeny*
RNA, Ribosomal, 18S / genetics
Sequence Analysis, DNA

Substances

RNA, Ribosomal, 18S