Abstract
Aim:
The present study was designed to evaluate the effects of irinotecan hydrochloride (IRI)- or metformin hydrochloride (MET)-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for the treatment of glioblastoma multiforme using in vitro neuron and U-87 MG glioblastoma cell cultures and in vivo animal model.
Methods:
The cytotoxic and neurotoxic effects of pure drugs, blank NPs and MET- and IRI-loaded PLGA NPs were investigated in vitro (using methylthiazolyldiphenyl-tetrazolium bromide assay) and in vivo (using Cavalieri's principle for estimation of cancer volume).
Results:
1 and 2 mM doses of MET and MET-loaded PLGA NPs, respectively, significantly reduced the volume of extracted cancer.
Conclusion:
Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach for the treatment of glioblastoma multiforme.
Keywords:
Cavalieri's principle; PLGA; brain tumor; irinotecan; metformin; nanoparticles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Combined Chemotherapy Protocols / administration & dosage
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Antineoplastic Combined Chemotherapy Protocols / chemistry
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cell Survival / drug effects
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Drug Carriers / administration & dosage
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Drug Carriers / chemistry
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Glioblastoma / drug therapy*
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Glioblastoma / pathology
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Humans
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Irinotecan / administration & dosage*
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Irinotecan / chemistry
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Metformin / administration & dosage*
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Metformin / chemistry
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Microscopy, Electron, Transmission
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Nanoparticles / administration & dosage*
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Nanoparticles / chemistry
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Neoplasms, Experimental / drug therapy*
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Neoplasms, Experimental / pathology
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Neurons / drug effects
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Neurons / pathology
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Polylactic Acid-Polyglycolic Acid Copolymer / administration & dosage
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Polylactic Acid-Polyglycolic Acid Copolymer / chemistry
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Rats
Substances
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Drug Carriers
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Polylactic Acid-Polyglycolic Acid Copolymer
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Irinotecan
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Metformin