Failure of a multiplex polymerase chain reaction assay to detect IMP-27 in a clinical isolate of Morganella morganii

Andrew Walkty; Heather Adam; Vanessa Tran; David C Alexander; David Boyd; Amrita Bharat; James Karlowsky

doi:10.1016/j.diagmicrobio.2018.06.011

Failure of a multiplex polymerase chain reaction assay to detect IMP-27 in a clinical isolate of Morganella morganii

Diagn Microbiol Infect Dis. 2018 Nov;92(3):194-195. doi: 10.1016/j.diagmicrobio.2018.06.011. Epub 2018 Jun 22.

Authors

Andrew Walkty¹, Heather Adam², Vanessa Tran³, David C Alexander⁴, David Boyd⁵, Amrita Bharat⁵, James Karlowsky²

Affiliations

¹ Department of Medical Microbiology & Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada; Diagnostic Services Manitoba, Winnipeg, Manitoba, Canada. Electronic address: awalkty@sharedhealthmb.ca.
² Department of Medical Microbiology & Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada; Diagnostic Services Manitoba, Winnipeg, Manitoba, Canada.
³ Department of Medical Microbiology & Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada.
⁴ Department of Medical Microbiology & Infectious Diseases, Max Rady College of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada; Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
⁵ National Microbiology Laboratory, Winnipeg, Manitoba, Canada.

PMID: 30025967
DOI: 10.1016/j.diagmicrobio.2018.06.011

Abstract

A case of an IMP-27-positive Morganella morganii isolate is reported, where the carbapenemase enzyme was demonstrated by whole genome sequencing. Carbapenemase detection using a multiplex PCR assay was negative due to mutations in the primer binding site. This case serves to illustrate the limitations of multiplex PCR for carbapenemase detection.

Keywords: Carbapenemase; Gram-negative bacilli; IMP-27.

MeSH terms

Enterobacteriaceae Infections / diagnosis*
Enterobacteriaceae Infections / microbiology*
Humans
Morganella morganii / enzymology
Morganella morganii / genetics*
Morganella morganii / isolation & purification
Multiplex Polymerase Chain Reaction / standards*
Mutation
Reproducibility of Results
Sensitivity and Specificity
beta-Lactamases / genetics*

Substances

beta-Lactamases