Microbes accumulate heavy metals after adsorption or absorption. This study exhibited that Trametes versicolor can tolerate up to 5 mg/g concentration of cadmium. Change in fungus morphology due to cadmium along with its absorption were analyzed using SEM, XRD, and EDAX. Cadmium absorption usually increased with time, and it was determined quantitatively by a fluorimetry technique using a synthesized imine fluorophore as a specific probe and compared with results obtained from atomic absorption spectroscopy (AAS). The intensity of the cadmium-specific XRD peak gradually increased up to the seventh day, and the absorption by the organisms reduced the concentration of cadmium even from the effluent of the plating industry. After the seventh day, Trametes versicolor absorbed almost 0.300 mg/g concentration of cadmium as visualized under high content screening from the fluorescence appearance of hyphae. Hence it can be concluded that Trametes versicolor may play a key role in reducing cadmium from a contaminated environment.