Printed Combinatorial Sensors for Simultaneous Detection of Ascorbic Acid, Uric Acid, Dopamine, and Nitrite

Chun-Hao Su; Chia-Liang Sun; Ying-Chih Liao

doi:10.1021/acsomega.7b00681

Printed Combinatorial Sensors for Simultaneous Detection of Ascorbic Acid, Uric Acid, Dopamine, and Nitrite

ACS Omega. 2017 Aug 31;2(8):4245-4252. doi: 10.1021/acsomega.7b00681. Epub 2017 Aug 4.

Authors

Chun-Hao Su¹, Chia-Liang Sun^{2

2

3}, Ying-Chih Liao¹

Affiliations

¹ Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
² Department of Chemical and Materials Engineering and Biosensor Group, Biomedical Engineering Research Center, Chang Gung University, Taoyuan 33302, Taiwan.
³ Department of Chemical and Materials Engineering, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan.

Abstract

In this study, an effective and simple direct printing method was developed to create sensing devices on screen-printed carbon electrodes (SPCEs) to detect multiple species simultaneously. Two sensing materials, graphene oxide nanoribbons (GONRs) and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS), were printed on one SPCE for detection of multiple biochemical substances. Printed layers of the GONRs and PEDOT:PSS mixture (GONRs & PEDOT:PSS) on SPCE showed embedment of GONRs in the PEDOT:PSS layer and diminished the electrochemical activity of GONRs. In contrast, by printing the GONRs and PEDOT:PSS at separate locations (GONRs + PEDOT:PSS) on the same SPCE, the electrochemical activities of both GONRs and PEDOT:PSS can be preserved. Thus, without synthesizing new materials, the modified electrode is able to simultaneously detect ascorbic acid (AA), uric acid (UA), dopamine (DA), and nitrite (NO₂^-), with high anodic oxidation currents and well-separated voltammetric peaks, in differential pulse voltammetry measurements. The detection limits for the four analytes are 41 nM (AA), 30 nM (DA), 11 nM (UA), and 18 nM (NO₂^-), respectively. The electrode can either detect single species separately or simultaneously determine specific concentrations of the four species in aqueous mixtures, and this can be further extended for many other electrochemical sensing applications.