DNA strand displacement plays an important role in biological computations. The inherent advantages of parallelism, high storability, and cascading have resulted in increased functional circuit realization of DNA strand displacement on the nanoscale. Herein, we propose an analog computation with minus based on DNA strand displacement. The addition, subtraction, multiplication, and division gates as elementary gates could realize analog computation with minus. The advantages of this proposal are the analog computation with negative value and division computation. In this article, we provide the designs and principles of these elementary gates and demonstrate gate performance by simulation. Furthermore, to show the cascade property of gates, we computed a polynomial as an example by these gates.