Rapid and Easy Extracellular Vesicle Detection on a Surface-Functionalized Power-Free Microchip toward Point-of-Care Diagnostics

Ryo Ishihara; Tadaaki Nakajima; Yoshitaka Uchino; Asuka Katagiri; Kazuo Hosokawa; Mizuo Maeda; Yasuhiro Tomooka; Akihiko Kikuchi

doi:10.1021/acsomega.7b01147

Rapid and Easy Extracellular Vesicle Detection on a Surface-Functionalized Power-Free Microchip toward Point-of-Care Diagnostics

ACS Omega. 2017 Oct 31;2(10):6703-6707. doi: 10.1021/acsomega.7b01147. Epub 2017 Oct 12.

Authors

Ryo Ishihara¹, Tadaaki Nakajima¹, Yoshitaka Uchino¹, Asuka Katagiri¹, Kazuo Hosokawa², Mizuo Maeda², Yasuhiro Tomooka¹, Akihiko Kikuchi¹

Affiliations

¹ Department of Materials Science and Technology and Department of Biological Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585, Japan.
² Bioengineering Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.

Abstract

Extracellular vesicles (EVs) are promising novel cancer biomarkers. However, rapid and easy analysis of EVs is challenging because conventional detection methods require large sample volumes and long detection times. Microchip-based analytical systems have particularly attracted attention for development of point-of-care (POC) diagnostics. Previously, various biomarker detection methods on a portable power-free poly(dimethylsiloxane) (PDMS) microchip using laminar flow-assisted dendritic amplification have been developed. Recently, for easy functionalization, we proposed a microchannel inner surface-functionalized power-free PDMS microchip (SF-PF microchip) utilizing electron beam-induced graft polymerization. In this study, we apply the technique and prepare a novel SF-PF microchip. On the microchip, EVs were successfully detected. The required sample volume was 1.0 μL, and the total analysis time was 20 min. The microchip can contribute to EV-based POC cancer diagnosis.