Cry78Aa, a novel Bacillus thuringiensis insecticidal protein with activity against Laodelphax striatellus and Nilaparvata lugens

Yinglong Wang; Yonglei Liu; Jie Zhang; Neil Crickmore; Fuping Song; Jiguo Gao; Changlong Shu

doi:10.1016/j.jip.2018.07.007

Cry78Aa, a novel Bacillus thuringiensis insecticidal protein with activity against Laodelphax striatellus and Nilaparvata lugens

J Invertebr Pathol. 2018 Oct:158:1-5. doi: 10.1016/j.jip.2018.07.007. Epub 2018 Jul 11.

Authors

Yinglong Wang¹, Yonglei Liu², Jie Zhang³, Neil Crickmore⁴, Fuping Song³, Jiguo Gao⁵, Changlong Shu⁶

Affiliations

¹ School of Life Science, Northeast Agricultural University, Harbin 150030, PR China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
² State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; State Key Laboratory of Medical Vector Surveillance and Pathogen Detection, Beilun Entry and Exit Inspection and Quarantine Bureau, Ningbo 305012, PR China.
³ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
⁴ School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK.
⁵ School of Life Science, Northeast Agricultural University, Harbin 150030, PR China. Electronic address: gaojiguo1961@hotmail.com.
⁶ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China. Electronic address: clshu@ippcaas.cn.

PMID: 30017953
DOI: 10.1016/j.jip.2018.07.007

Abstract

Transgenic plants expressing insecticidal proteins originating from Bacillus thuringiensis (Bt) have successfully been used to control lepidopteran and coleopteran pests with chewing mouthparts. However, only a handful of Bt proteins have been identified that have bioactivity against sap sucking pests (Hemiptera), including aphids, whiteflies, plant bugs and planthoppers. A novel Bt insecticidal protein with significant toxicity against a hemipteran insect pest is described here. The gene encoding the 359 amino acid, 40.7 kDa protein was cloned from strain C9F1. After expression and purification of the toxin, its median lethal concentration (LC₅₀) values against Laodelphax striatellus and Nilaparvata lugens were determined as 6.89 μg/mL and 15.78 μg/mL respectively. Analysis of the toxin sequence revealed the presence of both Toxin_10 and Ricin_B_Lectin domains.

Keywords: Hemiptera; Insecticidal protein; Planthopper.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacillus thuringiensis / genetics*
Bacterial Toxins / genetics*
Hemiptera*
Pest Control, Biological / methods*
Plants, Genetically Modified / genetics*

Substances

Bacterial Toxins