Sequencing Mirror-Image DNA Chemically

Xianyu Liu; Ting F Zhu

doi:10.1016/j.chembiol.2018.06.005

Sequencing Mirror-Image DNA Chemically

Cell Chem Biol. 2018 Sep 20;25(9):1151-1156.e3. doi: 10.1016/j.chembiol.2018.06.005. Epub 2018 Jul 12.

Authors

Xianyu Liu¹, Ting F Zhu²

Affiliations

¹ School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Center for Synthetic and Systems Biology, Ministry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China.
² School of Life Sciences, Tsinghua-Peking Center for Life Sciences, Center for Synthetic and Systems Biology, Ministry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing 100084, China. Electronic address: tzhu@tsinghua.edu.cn.

PMID: 30017914
DOI: 10.1016/j.chembiol.2018.06.005

Abstract

The development of mirror-image biology systems faces a crucial barrier of lacking an L-DNA sequencing technique. Here, we developed a practical method for sequencing mirror-image DNA by adopting the Maxam-Gilbert sequencing approach, through which specific nucleobases in an end-labeled L-DNA are cleaved by achiral chemicals. This technique may facilitate the therapeutic application of nuclease-resistant L-aptamer drugs, and bring the vision of building an alternative, mirror-image self-replicating system closer to reality.

Keywords: L-DNA; L-aptamer; Maxam-Gilbert sequencing; chirality.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide / chemistry*
Base Sequence
DNA / chemistry*
Sequence Analysis, DNA / methods*
Stereoisomerism

Substances

Aptamers, Nucleotide
DNA