Immunomics analysis of Babesia microti protein markers by high-throughput screening assay

Xia Zhou; Ji-Lei Huang; Hai-Mo Shen; Bin Xu; Jun-Hu Chen; Xiao-Nong Zhou

doi:10.1016/j.ttbdis.2018.07.004

Immunomics analysis of Babesia microti protein markers by high-throughput screening assay

Ticks Tick Borne Dis. 2018 Sep;9(6):1468-1474. doi: 10.1016/j.ttbdis.2018.07.004. Epub 2018 Jul 4.

Authors

Xia Zhou¹, Ji-Lei Huang², Hai-Mo Shen³, Bin Xu⁴, Jun-Hu Chen⁵, Xiao-Nong Zhou⁶

Affiliations

¹ Medical School of Soochow University, No. 199 Renai Road, Suzhou 215123, People's Republic of China; National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Shanghai 200025, People's Republic of China. Electronic address: zhouxia@suda.edu.cn.
² National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Shanghai 200025, People's Republic of China. Electronic address: Huangjilei1988@163.com.
³ National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Shanghai 200025, People's Republic of China. Electronic address: kasumi1979@tom.com.
⁴ National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Shanghai 200025, People's Republic of China. Electronic address: xubin677@hotmail.com.
⁵ National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Shanghai 200025, People's Republic of China. Electronic address: chenjh@nipd.chinacdc.cn.
⁶ National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Center for Tropical Diseases, National Center for International Research on Tropical Diseases, Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, Shanghai 200025, People's Republic of China. Electronic address: ipdzhouxn@sh163.net.

PMID: 30017725
DOI: 10.1016/j.ttbdis.2018.07.004

Abstract

Babesia microti is a protozoan considered to be a major etiological agent of emerging human babesiosis. It imposes an increasing public-health threat and can be overlooked because of low parasitemia or mixed infection with other pathogens. More sensitive and specific antigens are needed to improve the diagnosis of babesiosis. To screen the immune diagnostic antigens of B. microti, 204 sequences from homologue proteins between B. microti and B. bovis genome sequences in PiroplasmaDB were selected. The high throughput cloned and expressed B. microti proteins were screened with the sera from the BALB/c mice infected by B. microti using protein arrays. Ten (5.9%, 10/169) highly immunoreactive proteins were identified, and most (80%, 8/10) of these highly immunoreactive proteins had not been characterized before, making them potentially useful as candidate antigens for the development of diagnostic tools for babesiosis.

Keywords: Antigens; Babesia microti; Immunoproteomics; Protein microarray.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Protozoan / immunology*
Babesia microti / genetics*
Babesiosis / immunology*
Biomarkers / analysis
Genome, Protozoan / genetics*
Kinetics
Mice, Inbred BALB C
Protein Array Analysis
Rats

Substances

Antigens, Protozoan
Biomarkers