Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain

Hans Wijaya; Kengo Sasaki; Prihardi Kahar; Yopi; Hideo Kawaguchi; Takashi Sazuka; Chiaki Ogino; Bambang Prasetya; Akihiko Kondo

doi:10.1016/j.biortech.2018.07.039

Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain

Bioresour Technol. 2018 Oct:265:542-547. doi: 10.1016/j.biortech.2018.07.039. Epub 2018 Jul 7.

Authors

Hans Wijaya¹, Kengo Sasaki², Prihardi Kahar³, Yopi⁴, Hideo Kawaguchi², Takashi Sazuka⁵, Chiaki Ogino⁶, Bambang Prasetya⁷, Akihiko Kondo⁸

Affiliations

¹ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, Cibinong, 16911 Bogor, West Java, Indonesia.
² Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
³ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
⁴ Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, Cibinong, 16911 Bogor, West Java, Indonesia.
⁵ Bioscience and Biotechnology Center, Nagoya University, Furocho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
⁶ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. Electronic address: ochiaki@port.kobe-u.ac.jp.
⁷ National Standardization Agency of Indonesia (BSN), Gedung Badan Pengkajian Dan Penerapan Teknologi (BPPT), Jl. M.H. Thamrin No. 8, Jakarta 10340, Indonesia.
⁸ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.

PMID: 30017362
DOI: 10.1016/j.biortech.2018.07.039

Abstract

The aim of this study was to construct a cost-effective method for repeated bioethanol production using membrane (ultrafiltration permeation and nanofiltration concentration)-concentrated sweet sorghum juice by using flocculent Saccharomyces cerevisiae F118 strain. With low initial dry cell concentrations at around 0.28-0.35 g L^-1, the S. cerevisiae F118 strain provided an ethanol titer of 86.19 ± 1.15 g L^-1 (theoretical ethanol yield of 70.77%), which was higher than the non-flocculent S. cerevisiae BY4741 strain at 33.92 ± 0.99 g L^-1 after 24 h fermentation. This result was correlated with higher gene expressions of the sucrose-hydrolysing enzyme invertase, sugar phosphorylation, and pyruvate-to-ethanol pathways in the F118 strain compared with the BY4741 strain. Sequential fed-batch fermentation was conducted, and the F118 strain was easily separated from the fermentation broth via the formation of flocs and sediment. After the 5th cycle of fermentation with the F118 strain, the ethanol concentration reached 100.37 g L^-1.

Keywords: Bioethanol; Flocculent Saccharomyces cerevisiae; Gene expression; Membrane technology; Sweet sorghum juice.

MeSH terms

Edible Grain
Ethanol / chemistry*
Fermentation*
Flocculation
Saccharomyces cerevisiae
Sorghum*

Substances

Ethanol