[Interference of Lumican Regulates the Invasion and Migration of Liver Cancer Cells]

Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 May;49(3):358-363.
[Article in Chinese]

Abstract

Objective: To explore the effect of silencing lumican on the invasion and migration of liver cancer cells.

Methods: Lumican was silenced by shRNA in liver cancer cells (HepG2 and MHCC97H). The mRNA levels of lumican were detected by qRT-PCR. Cell invasion was measured by Transwell. Cell migration was tested via wound healing. The protein levels of lumican,MMP-9,VEGF,ERK1,JNK,p-ERK1 and p-JNK were measured by Western blot.

Results: Liver cancer cells (HepG2 and MHCC97H) had higher levels of mRNA and protein of lumican compared with normal hepatocyte L02 (P<0.01). shRNA lowered the levels of mRNA and protein of lumican (P<0.01),and weakened the invasion and migration of cancer cells (P<0.01). The expressions of MMP-9 and VEGF decreased with the shRNA silence (P<0.01). shRNA also reduced the protein level of p-ERK1 and p-JNK (P<0.01).

Conclusion: Silencing lumican by shRNA attenuates the invasion and migration of liver cancer cells via inhibiting the activation of ERK1/JNK pathway.

Keywords: ERK1/JNK pathway; Invasion; Liver cancer; Migration; lumican; shRNA.

MeSH terms

  • Cell Line, Tumor
  • Cell Movement*
  • Gene Silencing*
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / pathology*
  • Lumican / genetics
  • Lumican / metabolism*
  • MAP Kinase Kinase 4 / metabolism
  • Matrix Metalloproteinase 9 / metabolism
  • Mitogen-Activated Protein Kinase 3 / metabolism
  • Neoplasm Invasiveness*
  • RNA, Small Interfering
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Lumican
  • RNA, Small Interfering
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • Mitogen-Activated Protein Kinase 3
  • MAP Kinase Kinase 4
  • MMP9 protein, human
  • Matrix Metalloproteinase 9