A dose-response study of the bioavailability of grape seed proanthocyanidin in rat and lipid-lowering effects of generated metabolites in HepG2 cells

Maria Margalef; Ligia Guerrero; Zara Pons; Francisca Isabel Bravo; Lluís Arola; Begoña Muguerza; Anna Arola-Arnal

doi:10.1016/j.foodres.2014.07.019

A dose-response study of the bioavailability of grape seed proanthocyanidin in rat and lipid-lowering effects of generated metabolites in HepG2 cells

Food Res Int. 2014 Oct:64:500-507. doi: 10.1016/j.foodres.2014.07.019. Epub 2014 Aug 1.

Authors

Maria Margalef¹, Ligia Guerrero², Zara Pons¹, Francisca Isabel Bravo¹, Lluís Arola³, Begoña Muguerza⁴, Anna Arola-Arnal¹

Affiliations

¹ Nutrigenomic Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Tarragona 43007, Spain.
² Nutrigenomic Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Tarragona 43007, Spain; Department of Research, Nutrition and Innovation, ALPINA S.A., Bogotá, Colombia.
³ Nutrigenomic Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Tarragona 43007, Spain; Centre Tecnològic de Nutrició i Salut (CTNS), TECNIO, CEICS, Reus 43204, Spain.
⁴ Nutrigenomic Group, Department of Biochemistry and Biotechnology, Rovira i Virgili University, Tarragona 43007, Spain; Centre Tecnològic de Nutrició i Salut (CTNS), TECNIO, CEICS, Reus 43204, Spain. Electronic address: begona.muguerza@urv.cat.

PMID: 30011680
DOI: 10.1016/j.foodres.2014.07.019

Abstract

Hyperlipidemia is one of the principal causes of cardiovascular disease and proanthocyanidins (PAs) regulate lipid homeostasis. This study aims to evaluate the concentration of PAs in rat serum after the administration of different doses of PAs and to determine the capacity of these metabolites to reduce de novolipid synthesis in HepG2 cells. Two hours after oral administration of different doses of a grape seed proanthocyanidin extract (GSPE) (1000, 375, 250 and 125mg/kg), serum was semi-purified and characterised by HPLC-ESI-MS/MS before analysing the synthesis and secretion of lipids in HepG2 cells. Results showed a dose-dependent appearance of metabolised PAs in serum at doses up to 375mg/kg and saturation at 1000mg/kg of GSPE. A reduction in cholesterol esters (CE), free cholesterol (FC) and triglycerides (TG) synthesis was observed without dose-dependence when the cells were treated with PAs metabolites. Moreover, a low dose of metabolites (125mg/kg) was sufficient to reduce FC and TG synthesis. In conclusion, the study demonstrated that PAs metabolise in a dose-dependent manner up to 370mg/kg but not dose-dependent effect was shown in reducing the de novosynthesis of lipids.

Keywords: Cholesterol; HPLC–MS/MS; Polyphenols; Proanthocyanidins; Triglycerides.