Active Induction of Experimental Autoimmune Encephalomyelitis (EAE) with MOG35-55 in the Mouse

Methods Mol Biol. 2018:1791:227-232. doi: 10.1007/978-1-4939-7862-5_17.

Abstract

Experimental autoimmune encephalomyelitis (EAE) is one of the most popular animal models of multiple sclerosis (MS). There are a number of EAE models, being actively induced EAE in strains such as C57Bl/6 mice very robust and reproducible. We herewith present details of the materials and methods for active EAE. Mice are immunized with an emulsion of myelin oligodendrocyte glycoprotein peptide 35-55 (MOG35-55) + complete Freund's adjuvant (supplemented with Mycobacterium tuberculosis), and treated with Bordetella pertussis toxin, to induce EAE. Sham-EAE mice are immunized with bovine serum albumin instead of MOG35-55.

Keywords: EAE; Experimental autoimmune encephalomyelitis; MOG35–55; Multiple sclerosis; Myelin oligodendrocyte glycoprotein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Disease Models, Animal
  • Encephalomyelitis, Autoimmune, Experimental / diagnosis
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Immunization
  • Mice
  • Multiple Sclerosis / immunology
  • Myelin-Oligodendrocyte Glycoprotein / chemistry
  • Myelin-Oligodendrocyte Glycoprotein / immunology*
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology*
  • Pertussis Toxin / adverse effects
  • Pertussis Toxin / immunology
  • Phenotype
  • Spinal Cord / metabolism
  • Symptom Assessment

Substances

  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • Pertussis Toxin