Temporal evolution of Acinetobacter baumannii ST107 clone: conversion of blaOXA-143 into blaOXA-231 coupled with mobilization of ISAba1 upstream occAB1

Res Microbiol. 2019 Jan-Feb;170(1):53-59. doi: 10.1016/j.resmic.2018.07.001. Epub 2018 Jul 9.

Abstract

Nine carbapenem-resistant Acinetobacter baumannii isolates carrying blaOXA-231 and an ISAba1 upstream occAB1 were evaluated. They were clonally related and belonged to ST107. An OXA-143-producing A. baumannii ST107 strain (Ac-148) that did not possess ISAba1 upstream occAB1 was included in the analysis. Reduction in the expression of occAB1 and a 4-fold increase of carbapenem MICs were observed for all isolates, except for the Ac-148 strain, probably due to the presence of ISAba1 upstream occAB1 but in the same transcriptional orientation. We reported an A. baumannii ST107 clone carrying blaOXA-143 that acquired a mutation resulting into blaOXA-231 and mobilized ISAba1 upstream occAB1.

Keywords: Antimicrobial resistance; Clone evolution; Insertion sequence; Multidrug-resistant pathogen; Outer membrane protein; β-lactamase.

MeSH terms

  • Acinetobacter Infections / microbiology*
  • Acinetobacter baumannii / drug effects
  • Acinetobacter baumannii / enzymology*
  • Acinetobacter baumannii / genetics*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • DNA Transposable Elements*
  • Drug Resistance, Multiple, Bacterial
  • Humans
  • Microbial Sensitivity Tests
  • Mutation
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • beta-Lactamases