Abstract
Protein G is present in group G streptococcus strain (G4223); the IgG-binding part of this protein contains three IgG-binding domains and binds human IgG with very high activity. We obtained two recombinant polypeptides G4223 and G14223 with high IgG-binding activity. Polypeptide G14223 consisting of three IgG-binding domains and W region has higher molecular weight and is characterized by higher affinity for IgG than polypeptide G4223 consisting of only three IgG-binding domains. It was shown that polypeptide affinity depends on its structure and size.
Keywords:
IgG-binding domain; IgG-binding polypeptide; group G streptococcus.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / immunology
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Base Sequence
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Binding Sites
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Cloning, Molecular
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Humans
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Immunoglobulin G / chemistry*
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Immunoglobulin G / genetics
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Immunoglobulin G / immunology
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Molecular Weight
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Peptides / chemistry*
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Peptides / genetics
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Peptides / immunology
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Plasmids / chemistry
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Plasmids / metabolism
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Protein Binding
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Protein Interaction Domains and Motifs
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Sequence Alignment
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Sequence Homology, Amino Acid
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Streptococcus gordonii / chemistry*
Substances
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Bacterial Proteins
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IgG Fc-binding protein, Streptococcus
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Immunoglobulin G
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Peptides
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Recombinant Proteins