Expression of New Recombinant IgG-Binding Polypeptides and Analysis of Their Capacity to Bind Human IgG

Bull Exp Biol Med. 2018 Jul;165(3):373-377. doi: 10.1007/s10517-018-4173-z. Epub 2018 Jul 12.

Abstract

Protein G is present in group G streptococcus strain (G4223); the IgG-binding part of this protein contains three IgG-binding domains and binds human IgG with very high activity. We obtained two recombinant polypeptides G4223 and G14223 with high IgG-binding activity. Polypeptide G14223 consisting of three IgG-binding domains and W region has higher molecular weight and is characterized by higher affinity for IgG than polypeptide G4223 consisting of only three IgG-binding domains. It was shown that polypeptide affinity depends on its structure and size.

Keywords: IgG-binding domain; IgG-binding polypeptide; group G streptococcus.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / immunology
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression
  • Humans
  • Immunoglobulin G / chemistry*
  • Immunoglobulin G / genetics
  • Immunoglobulin G / immunology
  • Molecular Weight
  • Peptides / chemistry*
  • Peptides / genetics
  • Peptides / immunology
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Streptococcus gordonii / chemistry*

Substances

  • Bacterial Proteins
  • IgG Fc-binding protein, Streptococcus
  • Immunoglobulin G
  • Peptides
  • Recombinant Proteins