Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings

H F Ashpitel; E B Dabbs; J L Nemchand; R M La Ragione; F J Salguero; M S Whiteley

doi:10.1016/j.ejvssr.2018.03.003

Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings

EJVES Short Rep. 2018 Apr 18:39:7-11. doi: 10.1016/j.ejvssr.2018.03.003. eCollection 2018.

Authors

H F Ashpitel^{1

2}, E B Dabbs¹, J L Nemchand^{1

2}, R M La Ragione², F J Salguero², M S Whiteley^{1

2}

Affiliations

¹ The Whiteley Clinic, Stirling House, Guildford, UK.
² Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.

Abstract

Objectives: To analyse the biological effects of a 1920 nm endovenous laser (EVL) on extra-fascial great saphenous vein (GSV) in vitro.

Methods: A 10 cm length of a large tributary bypassing a hypoplastic segment of the GSV (sometimes called an "extra-fascial GSV") was obtained during routine varicose vein surgery. The length was treated in five sections with different LEEDs (0 (control), 20, 40, 60, and 80 J/cm) with a 1920 nm EVL at 4W power, in a novel in vitro treatment model. The biological effects were assessed by histological staining of the samples for haematoxylin and eosin (HE) and Martius Scarlet Blue (MSB), and by immunofluorescent detection of p-p53 and VCAM-1.

Results: Histological analysis showed significant structural damage at LEEDs above 60 J/cm, especially in the intima and media, with the treatment at 80 J/cm causing perforation of the vein wall. In addition, there was a significant increase in p-p53 expression in treated tissue at 60 and 80 J/cm.

Conclusions: Using this ex vivo model, the results indicate that in vitro treatment with a 1920 nm EVL, at or above an LEED of 60 J/cm and 4 W power, causes significant vein wall cell death reaching deep into the media by a combination of direct thermal damage and apoptosis. A wavelength of 1920 nm appears to be effective for the endovenous ablation of truncal veins.

Keywords: Endovenous laser treatment; Endovenous thermal ablation; Great saphenous vein; Histology; Immunofluorescence.