On-chip light-sheet fluorescence imaging flow cytometry at a high flow speed of 1 m/s

Taichi Miura; Hideharu Mikami; Akihiro Isozaki; Takuro Ito; Yasuyuki Ozeki; Keisuke Goda

doi:10.1364/BOE.9.003424

On-chip light-sheet fluorescence imaging flow cytometry at a high flow speed of 1 m/s

Biomed Opt Express. 2018 Jun 27;9(7):3424-3433. doi: 10.1364/BOE.9.003424. eCollection 2018 Jul 1.

Authors

Taichi Miura¹, Hideharu Mikami^{1

2}, Akihiro Isozaki¹, Takuro Ito³, Yasuyuki Ozeki⁴, Keisuke Goda^{1

3

5}

Affiliations

¹ Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
² mikami@chem.s.u-tokyo.ac.jp.
³ Japan Science and Technology Agency, Saitama 332-0012, Japan.
⁴ Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo 113-8656, Japan.
⁵ goda@chem.s.u-tokyo.ac.jp.

Abstract

We present on-chip fluorescence imaging flow cytometry by light-sheet excitation on a mirror-embedded microfluidic chip. The method allows us to obtain microscopy-grade fluorescence images of cells flowing at a high speed of 1 m/s, which is comparable to the flow speed of conventional non-imaging flow cytometers. To implement the light-sheet excitation of flowing cells in a microchannel, we designed and fabricated a mirror-embedded PDMS-based microfluidic chip. To show its broad utility, we used the method to classify large populations of microalgal cells (Euglena gracilis) and human cancer cells (human adenocarcinoma cells). Our method holds promise for large-scale single-cell analysis.

Keywords: (110.0110) Imaging systems; (170.0170) Medical optics and biotechnology; (180.0180) Microscopy.