Dual-functional probe based on rhodamine for sequential Cu2+ and ATP detection in vivo

Xilang Jin; Jingkai Gao; Pu Xie; Mengchen Yu; Ting Wang; Hongwei Zhou; Aijie Ma; Qian Wang; Xin Leng; Xianghan Zhang

doi:10.1016/j.saa.2018.06.094

Dual-functional probe based on rhodamine for sequential Cu²⁺ and ATP detection in vivo

Spectrochim Acta A Mol Biomol Spectrosc. 2018 Nov 5:204:657-664. doi: 10.1016/j.saa.2018.06.094. Epub 2018 Jun 30.

Authors

Xilang Jin¹, Jingkai Gao², Pu Xie³, Mengchen Yu⁴, Ting Wang³, Hongwei Zhou⁵, Aijie Ma³, Qian Wang³, Xin Leng⁶, Xianghan Zhang⁷

Affiliations

¹ School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, 710032, Shaanxi, China. Electronic address: jinxilang_911@163.com.
² School of Life Sciences and Technology, Xidian University, Xi'an, 710071, Shaanxi, China.
³ School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, 710032, Shaanxi, China.
⁴ State and Local Joint Engineering Lab. of Advanced Network and Monitoring Controls, Xi'an Technological University, Xi'an, 710032, Shaanxi, China.
⁵ School of Materials and Chemical Engineering, Xi'an Technological University, Xi'an, 710032, Shaanxi, China. Electronic address: xatuzhou@163.com.
⁶ Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecule Chemistry, College of Chemistry & Materials Science, Northwest University, Xi'an, Shaanxi 710069, China.
⁷ School of Life Sciences and Technology, Xidian University, Xi'an, 710071, Shaanxi, China. Electronic address: xhzhang@xidian.edu.cn.

PMID: 29982156
DOI: 10.1016/j.saa.2018.06.094

Abstract

A rhodamine-based fluorescent probe for Cu²⁺ and ATP has been designed. The fluorescence intensity/absorbance was significantly enhanced upon the addition of Cu²⁺ owning to the opening of the spiro-ring of rhodamine, which quickly returned to the original level due to the reconstruction of the probe by the reacting with ATP. Cu²⁺/ATP-induced fluorescent intensity/aborbance changes showed a good linear relationship with the concentration of Cu²⁺/ATP in the range of 2-20 μM/0-10 μM with a detection limit of 0.1 μM/1.0 μM. The proposed method is simple in design and fast in operation, and is suitable for the reversible monitoring of Cu²⁺ and ATP in bioanalytical applications.

Keywords: ATP; Bioimaging; Cu(2+); Fluorescence; Rhodamine.

MeSH terms

Adenosine Triphosphate / analysis*
Animals
Copper / analysis*
Fluorescent Dyes / chemistry*
Limit of Detection
Linear Models
Mice
Mice, Nude
Optical Imaging
Reproducibility of Results
Rhodamines / chemistry*
Spectrometry, Fluorescence
Water Pollutants, Chemical / analysis

Substances

Fluorescent Dyes
Rhodamines
Water Pollutants, Chemical
Copper
Adenosine Triphosphate