Modulatory effects of taurine on metabolic and oxidative stress parameters in a mice model of muscle overuse

Anand Thirupathi; Sharon Freitas; Helen R Sorato; Giulia S Pedroso; Pauline S Effting; Adriani P Damiani; Vanessa M Andrade; Renata T Nesi; Ramesh C Gupta; Alexandre P Muller; Ricardo A Pinho

doi:10.1016/j.nut.2018.03.058

Modulatory effects of taurine on metabolic and oxidative stress parameters in a mice model of muscle overuse

Nutrition. 2018 Oct:54:158-164. doi: 10.1016/j.nut.2018.03.058. Epub 2018 Apr 25.

Affiliations

¹ Laboratory of Exercise Biochemistry and Physiology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil.
² Laboratory of Molecular and Cellular Biology, Graduate Program in Health Sciences, Health Sciences Unit, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil.
³ Nagaland University, Nagaland, India.
⁴ Laboratory of Exercise Biochemistry in Health, School of Medicine, Graduate Program in Health Science, Pontifícia Universidade Católica do Paraná, Curitiba, Paraná, Brazil. Electronic address: Rapinho12@gmail.com.

PMID: 29982143
DOI: 10.1016/j.nut.2018.03.058

Abstract

Objective: The aim of this study was to investigate the regulatory effects of taurine on the biochemical parameters of muscle injury by overuse.

Methods: Male Swiss mice were divided into four groups: control (Ctrl), overuse (Ov), taurine (Tau), and overuse plus taurine (OvTau). High-intensity exercise sessions were administered for 21 d with concomitant subcutaneous injections of taurine (150 mg/kg). The mice were then sacrificed. The quadriceps muscles were surgically removed for subsequent histologic analysis and evaluation of mitochondrial function, oxidative stress parameters, tissue repair, and DNA damage markers.

Results: The Ov group showed significant differences compared with the Ctrl group (all P <0.05). The fiber area decreased by 49.34%, whereas the centralized nuclei contents (Ctrl = 1.33%; Ov = 28.67%), membrane potential (Ctrl_suc = 179.05 arbitrary fluorescence units (AFUs), Ctrl_suc+ADP = 198.11 AFUs; Ov_suc = 482.95 AFUs, Ov_suc+ADP = 461.6 AFUs), complex I activity (Ctrl = 20.45 nmol ⋅ min ⋅ mg protein, Ov = 45.25 nmol ⋅ min ⋅ mg protein), hydrogen peroxide (Ctrl_suc = 1.08 relative fluorescence unit (RFU) ⋅ sec ⋅ mg protein, Ctrl_suc+ADP = 0.23 RFU ⋅ sec ⋅ mg protein; Ov_suc = 5.02 RFU ⋅ sec ⋅ mg protein, Ov_suc+ADP = 0.26 RFU ⋅ sec ⋅ mg protein) and malondialdehyde (Ctrl = 0.03 nmol ⋅ mg ⋅ protein, Ov = 0.06 nmol ⋅ mg ⋅ protein) levels, and DNA damage (Ctrl_freq = 7.17%, Ov_freq = 31.17%; Ctrl_index = 4.17, Ov_index = 72.5) were increased. Taurine administration reduced the number of centralized nuclei (OvTau = 5%), hydrogen peroxide levels (OvTau_suc = 2.81 RFU ⋅ sec ⋅ mg protein, OvTaus_suc+ADP = 1.54 RFU ⋅ sec ⋅ mg protein), membrane potential (OvTau_suc = 220.18 AFUs, OvTaus_suc+ADP = 235.28 AFUs), lipid peroxidation (OvTau = 0.02 nmol/mg protein), and DNA damage (OvTau_freq = 21.33%, OvTau_index = 47.83) and increased the fiber area by 54% (all P <0.05).

Conclusion: Taken together, these data suggest that taurine supplementation modulates various cellular remodeling parameters after overuse-induced muscle damage, and that these positive effects may be related to its antioxidant capacity.

Keywords: Mitochondrial function; Mouse; Muscle damage; Muscle overuse; Strenuous exercise; Taurine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / pharmacology*
Cumulative Trauma Disorders / drug therapy*
Cumulative Trauma Disorders / physiopathology
Disease Models, Animal
Male
Mice
Mitochondria / drug effects
Muscle, Skeletal / drug effects*
Oxidative Stress / drug effects*
Physical Conditioning, Animal / physiology
Taurine / pharmacology*

Substances

Antioxidants
Taurine