Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision In Vitro

Agnieszka Kiełkowska; Adela Adamus; Rafal Baranski

doi:10.1007/978-1-4939-8594-4_21

Haploid and Doubled Haploid Plant Production in Carrot Using Induced Parthenogenesis and Ovule Excision In Vitro

Methods Mol Biol. 2018:1815:301-315. doi: 10.1007/978-1-4939-8594-4_21.

Authors

Agnieszka Kiełkowska¹, Adela Adamus², Rafal Baranski²

Affiliations

¹ Faculty of Biotechnology and Horticulture, Institute of Plant Biology and Biotechnology, University of Agriculture, Krakow, Poland. a.kielkowska@urk.edu.pl.
² Faculty of Biotechnology and Horticulture, Institute of Plant Biology and Biotechnology, University of Agriculture, Krakow, Poland.

PMID: 29981131
DOI: 10.1007/978-1-4939-8594-4_21

Abstract

Haploid plants have a gametophytic number of chromosomes (n) in the sporophyte. A doubled haploid (DH) plant results from doubling the chromosome set of a haploid plant, as a consequence a homozygosity plant is produced at every locus (true homozygous plant). DH plants are of great significance in breeding programs for the improvement of plants. Here we describe a protocol for the production of doubled haploid plants in carrot (Daucus carota L.) using parthenogenesis induced by wide pollination.

Keywords: Daucus carota; Doubled haploid; Ovule culture; Wide pollination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Plant / genetics
DNA, Plant / isolation & purification
Daucus carota / embryology*
Daucus carota / genetics
Flow Cytometry
Haploidy*
Isoenzymes / metabolism
Ovule / physiology*
Parthenogenesis*
Plant Breeding / methods*
Pollination
Polymerase Chain Reaction
Tissue Culture Techniques

Substances

DNA, Plant
Isoenzymes